Journal: Cell death & disease

Article Title: Dronedarone hydrochloride (DH) induces pancreatic cancer cell death by triggering mtDNA-mediated pyroptosis.

doi: 10.1038/s41419-024-07102-w

Figure Lengend Snippet: Fig. 6 DH induced mtDNA release and triggered cGAS-STING pathway. A Representative images of PANC1 cells after stained with dsDNA and MitoTracker. Green, MitoTracker; Red, dsDNA. Scale bar, 100 μm. B Determination of extracellular DNA value in the culture medium of PANC-1 cells after DH treatment. n = 3. C Representative images of Western Blot images of cGAS-STING pathway-related proteins in PANC-1 cells after different treatment. D, E Quantification of STING, p-IRF3 protein expressions in Fig. 6B. n = 3. F Determination of extracellular DNA value in the culture medium of PANC-1 cells after DH or DH combined ddC treatment. n = 6. G Representative images of Western Blot images of cGAS-STING pathway-related proteins in PANC-1 cells after DH or DH combined ddC treatment. H, I Quantification of STING, p-IRF3 protein expressions in Fig. 6F. n = 3. Data was shown as mean ± SD. Mutiple t-test are followed by Bonferoni correction was used. *p < 0.05.

Article Snippet: Goat anti-rabbit IgG (Alexa Fluor 555 Conjugate) (4413S), anti-p-IRF3 antibody (29047t), anti-IRF3 antibody (11904t), anti-STING antibody (13647t), anti-NLRP3 antibody (15101 s), anti-ASC antibody (67824t), and anti-IL-1β antibody were purchased from Cell Signaling Technology (MA, USA).

Techniques: Staining, Western Blot